Human Recombinant ROBO4 protein, His Tag (V2LY-0526-LY6572)

Name: Human Recombinant ROBO4 protein, His Tag
SKU: V2LY-0526-LY6572
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ROBO4 protein, His Tag consist of Amino Acid: 1-469 and predicts a molecular mass of 49 kDa.

Molecule Mass

49 kDa

Verified

HPLC

Sequence

Amino Acid: 1-469

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ROUNDABOUT GUIDANCE RECEPTOR 4

Function

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity).
Involved in the maintenance of endothelial barrier organization and function (PubMed:30455415).

Biological Process

Biological Process angiogenesisManual Assertion Based On ExperimentIGI:UniProtKB
Biological Process axon guidanceManual Assertion Based On ExperimentIBA:GO_Central
Biological Process dendrite self-avoidanceManual Assertion Based On ExperimentIBA:GO_Central
Biological Process establishment of endothelial barrierManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process homophilic cell adhesion via plasma membrane adhesion moleculesManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of cell migration1 PublicationNAS:UniProtKB

Cellular Location

axon
extracellular exosome
integral component of membrane
plasma membrane

Involvement in disease

Aortic valve disease 3 (AOVD3):
A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification, stenosis and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome. AOVD3 features are bicuspid aortic valve, aortic valve stenosis, and ascending aortic aneurysm. Some patients have atrial septal defects. AOVD3 inheritance is autosomal dominant with incomplete penetrance.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant ROBO4 protein, His Tag (CAT#: V2LY-0526-LY8839)

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Mouse Recombinant ROBO4 protein, His Tag (CAT#: V2LY-0526-LY8839)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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