EGR1 Antibodies

Structure of EGR1

The molecular weight of the protein encoded by the EGR1 gene is approximately 53-57 kDa. The specific value varies among different species, mainly due to the differences in amino acid composition and post-transcriptional modifications. The following table shows a comparison of the molecular characteristics of EGR1 protein in different species:

The EGR1 protein is composed of 533 amino acids. Its core structure consists of three concatenated Cys2-His2 type zinc finger modalities. These domains form stable spatial conformations through zinc ion coordination, enabling it to specifically recognize and bind to the GC-rich sequence in the promoter region of the target gene. Each zinc finger unit coordinates with zinc ions through conserved cysteine and histidine residues to form an independent DNA-binding unit, while the connection sequences between adjacent zinc fingers provide structural flexibility. This modular design enables EGR1 to act as an early response transcription factor, rapidly responding to extracellular signals and initiating the expression regulation of downstream genes.

Fig. 1 EGR1 protein domains and post-translational modifications.¹

Key structural properties of EGR1:

• Contains three highly conserved C2H2 -type zinc finger domains
• Each zinc finger is coordinated with zinc ions by cysteine and histidine residues
• Zinc finger module form helical structure
• Basic amino acid area is responsible for the verification and protein interactions

Functions of EGR1

The main function of the EGR1 gene is to act as an immediate early response transcription factor, participating in the regulation of cell stress, proliferation and differentiation. In addition, it also involves a variety of pathophysiological processes, including neural plasticity, immune regulation and tumorigenesis.

The activation of EGR1 is characterized by its rapidness and transitivity. Its expression level often significantly increases within tens of minutes after stimulation and then rapidly returns to the baseline. This dynamic expression pattern makes it a key molecular bridge connecting extracellular signals with long-term adaptive gene expression.

Applications of EGR1 and EGR1 Antibody in Literature

1. Wang, Bin, et al. "The role of the transcription factor EGR1 in cancer." Frontiers in oncology 11 (2021): 642547. https://doi.org/10.3389/fonc.2021.642547

The article indicates that Early growth response factor 1 (EGR1) is a transcription factor that participates in processes such as tissue damage and immune responses. Recent studies have found that it may regulate cell proliferation, invasion and angiogenesis in the occurrence and development of tumors. The specific mechanism remains to be clarified, providing a reference for clinical efficacy prediction and exploration of new targets.

2. Pan, Mingang, et al. "EGR1 suppresses HCC growth and aerobic glycolysis by transcriptionally downregulating PFKL." Journal of Experimental & Clinical Cancer Research 43.1 (2024): 35. https://doi.org/10.1186/s13046-024-02957-5

This study reveals that the expression of EGR1 is decreased in hepatocellular carcinoma. It inhibits tumor proliferation by suppressing aerobic glycolysis regulated by the PFKL gene. Animal and organoid experiments have confirmed that EGR1 gene therapy can inhibit tumor growth and enhance the efficacy of sorafenib, demonstrating its potential as a new target for liver cancer treatment.

3. Chen, Jian-Wen, et al. "Transient upregulation of EGR1 signaling enhances kidney repair by activating SOX9+ renal tubular cells." Theranostics 12.12 (2022): 5434. https://doi.org/10.7150/thno.73426

This study reveals that in the early stage of acute kidney injury, the transcription factor EGR1 is rapidly upregulated. It accelerates the proliferation and repair of renal tubular cells by directly binding to the Sox9 gene promoter, promoting SOX9 expression and activating the Wnt/β-catenin pathway. The deficiency of EGR1 can aggravate the damage, indicating that it is a potential therapeutic target for kidney protection.

4. Havis, Emmanuelle, and Delphine Duprez. "EGR1 transcription factor is a multifaceted regulator of matrix production in tendons and other connective tissues." International journal of molecular sciences 21.5 (2020): 1664.https://doi.org/10.3390/ijms21051664

This study reveals that the transcription factor EGR1 is expressed in various connective tissues such as tendons and cartilages, and its core function lies in regulating the generation of extracellular matrix. It is involved in the development, homeostasis and repair processes of tissues and is closely related to fibrotic lesions, and thus is regarded as a potential target for anti-fibrotic treatment.

5. Woodson, Caitlin M., and Kylene Kehn-Hall. "Examining the role of EGR1 during viral infections." Frontiers in Microbiology 13 (2022): 1020220. https://doi.org/10.3389/fmicb.2022.1020220

This study reveals that EGR1 is a multifunctional transcription factor that can be activated by various signals such as growth factors, cellular stress, and viral infections. As a convergence point of signal pathways, it plays a significant role in neural function and cancer. This article focuses on reviewing the direct and indirect regulatory roles of EGR1 in the replication process of DNA and RNA viruses.

Creative Biolabs: EGR1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality EGR1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom EGR1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our EGR1 antibodies, custom preparations, or technical support, contact us at email.