MRC1 Antibodies

Structure of MRC1

The protein encoded by the MRC1 gene is a type I transmembrane glycoprotein with a molecular weight of approximately 162 kDa. The molecular weight of this protein will show certain differences in different cell types due to the varying degrees of glycosylation modification.

This protein is composed of 1,439 amino acid residues, and its extracellular region contains multiple functional domains that jointly mediate ligand recognition. Its C-type lectin domain is a key region for recognizing specific carbohydrate ligands and can bind to sugar structures such as mannose in a calcium ion-dependent manner. This domain coordinates with calcium ions through conserved amino acid residues (such as Glu and Asn), and then interacts with sugar ligands. This mechanism is crucial for pathogen recognition and endocytosis.

Fig. 1 Schematic representation of Mrc1 and its cohesin crosslinks.¹

Key structural properties of MRC1:

• Complex multi-domain extracellular regions
• Characteristic C-type lectin recognition domain
• Transmembrane anchoring and intracellular signal transduction module

Functions of MRC1

The main function of the protein encoded by the MRC1 gene is to participate in immune recognition and endocytosis. Meanwhile, this protein also plays a key role in a variety of physiological processes, including immune regulation and tissue repair.

The ligand binding characteristics of this protein are manifested as a broad-spectrum carbohydrate recognition mode. Compared with the epitopes of antibodies that specifically recognize a single antigen, its mode of action is more inclined to the typical features of pattern recognition receptors - responding to the threat of multiple pathogens by recognizing specific molecular patterns. This localization makes it an important part of the innate immune defense system.

Applications of MRC1 and MRC1 Antibody in Literature

1. Huang, Linxi, et al. "Mrc1+ macrophage-derived IGF1 mitigates crystal nephropathy by promoting renal tubule cell proliferation via the AKT/Rb signaling pathway." Theranostics 14.4 (2024): 1764. https://doi.org/10.7150/thno.89174

This study found through single-cell sequencing that in glyalate-induced crystal nephropathy, Mrc1+ macrophages promote the proliferation of renal tubular cells by secreting IGF1 and activating the AKT/Rb signaling pathway, thereby alleviating kidney injury. This provides a potential therapeutic target for crystalline nephropathy.

2. Poyuan, et al. "Mrc1-dependent chromatin compaction represses DNA double-stranded break repair by homologous recombination upon replication stress." Frontiers in Cell and Developmental Biology 9 (2021): 630777. https://doi.org/10.3389/fcell.2021.630777

This study reveals that under replication stress, Mrc1 induces global chromatin compression in a way that is independent of its partner proteins. This structural change hinders DNA terminal excision enzymes from approaching the damage site, thereby inhibiting homologous recombination repair and maintaining genomic stability.

3. Gellon, Lionel, et al. "Mrc1 and Tof1 prevent fragility and instability at long CAG repeats by their fork stabilizing function." Nucleic acids research 47.2 (2019): 794-805. https://doi.org/10.1093/nar/gky1195

This study reveals that when replication forks encounter CAG repeat sequences, the "fork stabilization" function of Mrc1 (independent of its checkpoint function) is crucial for preventing DNA breaks. This protective effect is particularly crucial in long repeat sequences, while the role of Tof1 is more sequence-specific.

4. Yu, Keesun, et al. "Characterization of splenic MRC1hiMHCIIlo and MRC1loMHCIIhi cells from the monocyte/macrophage lineage of White Leghorn chickens." Veterinary research 51.1 (2020): 73. https://doi.org/10.1186/s13567-020-00795-9

Two subsets of monocytes/macrophages were found in the spleen of chickens in this study: MRC1loMHCIIhi cells are good at antigen presentation and secretion of inflammatory factors; MRC1hiMHCIIlo cells, on the other hand, possess stronger phagocytic, migratory and bactericidal capabilities, and their proportions increase under inflammatory conditions, with clear functional differentiation.

5. Shrestha, Sudikchya, et al. "Replisome-cohesin interactions provided by the Tof1-Csm3 and Mrc1 cohesion establishment factors." Chromosoma 132.2 (2023): 117-135. https://doi.org/10.1007/s00412-023-00797-4

This study reveals that Mrc1 and Tof1-Csm3 regulate the chromosomal cohesin complex through direct and multi-faceted protein-protein interactions, thereby directly promoting the establishment of sister chromatid adhesions during DNA replication. This function is independent of their known replication-related roles.

Creative Biolabs: MRC1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality MRC1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom MRC1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our MRC1 antibodies, custom preparations, or technical support, contact us at email.