Sign in or Register   Sign in or Register
  |  

Rabbit Anti-ACOX1 Recombinant Antibody (V2-179358) (CBMAB-A0659-YC)

Provided herein is a Rabbit monoclonal antibody against Human Acyl-CoA Oxidase 1. The antibody can be used for immunoassay techniques, such as ELISA, IF, IHC, WB.
See all ACOX1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-179358
Antibody Isotype
IgG
Application
ELISA, IF, IHC, WB

Basic Information

Immunogen
ACOX1 Mab is generated from mouse immunized with ACOX1 recombinant protein
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:1,000
WB1:100-1:500
IF(ICC)1:10-1:50
IHC1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2, 0.09% sodium azide
Buffer
PBS, pH7.2
Preservative
0.09% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acyl-CoA Oxidase 1
Introduction
ACOX1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in
Entrez Gene ID
51
UniProt ID
Q15067
Alternative Names
Acyl-CoA Oxidase 1; Palmitoyl-CoA Oxidase; Acyl-Coenzyme A Oxidase 1, Palmitoyl; Straight-Chain Acyl-CoA Oxidase; Acyl-CoA Oxidase 1, Palmitoyl; EC 1.3.3.6; ACOX;
Function
Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. First enzyme of the fatty acid beta-oxidation pathway.
Isoform 1: Shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length.
Isoform 2: Is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
Biological Process
Alpha-linolenic acid metabolic process
Cholesterol homeostasis
Fatty acid beta-oxidation using acyl-CoA oxidase
Fatty acid catabolic process
Fatty acid oxidation
Generation of precursor metabolites and energy
Hydrogen peroxide biosynthetic process
Lipid homeostasis
Lipid metabolic process
Peroxisome fission
Prostaglandin metabolic process
Protein localization
Regulation of metabolic process
Spermatogenesis
Very long-chain fatty acid beta-oxidation
Very long-chain fatty acid metabolic process
Cellular Location
Peroxisome
Involvement in disease
Adrenoleukodystrophy, pseudoneonatal (Pseudo-NALD): A peroxisomal single-enzyme disorder of fatty acid beta-oxidation, resulting in clinical manifestations that remind neonatal adrenoleukodystrophy. Clinical features include mental retardation, leukodystrophy, seizures, mild hepatomegaly, hearing deficit. Pseudo-NALD is characterized by increased plasma levels of very-long chain fatty acids, due to decreased or absent peroxisome acyl-CoA oxidase activity. Peroxisomes are intact and functioning.
Mitchell syndrome (MITCH): A disorder characterized by episodic demyelination, sensorimotor polyneuropathy, and sensorineural hearing loss.

Morita, A., Enokizono, T., Ohto, T., Tanaka, M., Watanabe, S., Takada, Y., ... & Takada, H. (2021). Novel ACOX1 mutations in two siblings with peroxisomal acyl-CoA oxidase deficiency. Brain and Development, 43(3), 475-481.

You, L., Chen, J., Liu, W., Xiang, Q., Luo, Z., Wang, W., ... & Wu, J. (2020). Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence, 11(1), 537-553.

Raas, Q., Saih, F. E., Gondcaille, C., Trompier, D., Hamon, Y., Leoni, V., ... & Savary, S. (2019). A microglial cell model for acyl-CoA oxidase 1 deficiency. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1864(4), 567-576.

Chen, X. F., Tian, M. X., Sun, R. Q., Zhang, M. L., Zhou, L. S., Jin, L., ... & Ye, D. (2018). SIRT 5 inhibits peroxisomal ACOX 1 to prevent oxidative damage and is downregulated in liver cancer. EMBO reports, 19(5), e45124.

Kim, S., & Kim, K. J. (2018). Structural insight into the substrate specificity of acyl-CoA oxidase1 from Yarrowia lipolytica for short-chain dicarboxylyl-CoAs. Biochemical and biophysical research communications, 495(2), 1628-1634.

Vamecq, J., Andreoletti, P., El Kebbaj, R., Saih, F. E., Latruffe, N., El Kebbaj, M., ... & Cherkaoui-Malki, M. (2018). Peroxisomal acyl-coA oxidase type 1: anti-inflammatory and anti-aging properties with a special emphasis on studies with LPS and argan oil as a model transposable to aging. Oxidative medicine and cellular longevity, 2018.

Zeng, J., Deng, S., Wang, Y., Li, P., Tang, L., & Pang, Y. (2017). Specific inhibition of Acyl-CoA oxidase-1 by an acetylenic acid improves hepatic lipid and reactive oxygen species (ROS) metabolism in rats fed a high fat diet. Journal of Biological Chemistry, 292(9), 3800-3809.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-ACOX1 Recombinant Antibody (V2-179358)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare