Human Recombinant ACOX1 protein, His Tag (V2LY-0526-LY1903)

Name: Human Recombinant ACOX1 protein, His Tag
SKU: V2LY-0526-LY1903
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ACOX1 protein, His Tag consist of Amino Acid: 1-660 and predicts a molecular mass of 76.7 kDa.

Molecule Mass

76.7 kDa

Sequence

Amino Acid: 1-660

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Oxidase 1

Function

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. First enzyme of the fatty acid beta-oxidation pathway.
Isoform 1: Shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length.
Isoform 2: Is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.

Biological Process

Alpha-linolenic acid metabolic process
Cholesterol homeostasis
Fatty acid beta-oxidation using acyl-CoA oxidase
Fatty acid catabolic process
Fatty acid oxidation
Generation of precursor metabolites and energy
Hydrogen peroxide biosynthetic process
Lipid homeostasis
Lipid metabolic process
Peroxisome fission
Prostaglandin metabolic process
Protein localization
Regulation of metabolic process
Spermatogenesis
Very long-chain fatty acid beta-oxidation
Very long-chain fatty acid metabolic process

Cellular Location

Peroxisome

Involvement in disease

Adrenoleukodystrophy, pseudoneonatal (Pseudo-NALD): A peroxisomal single-enzyme disorder of fatty acid beta-oxidation, resulting in clinical manifestations that remind neonatal adrenoleukodystrophy. Clinical features include mental retardation, leukodystrophy, seizures, mild hepatomegaly, hearing deficit. Pseudo-NALD is characterized by increased plasma levels of very-long chain fatty acids, due to decreased or absent peroxisome acyl-CoA oxidase activity. Peroxisomes are intact and functioning.
Mitchell syndrome (MITCH): A disorder characterized by episodic demyelination, sensorimotor polyneuropathy, and sensorineural hearing loss.

Morita, A., Enokizono, T., Ohto, T., Tanaka, M., Watanabe, S., Takada, Y., ... & Takada, H. (2021). Novel ACOX1 mutations in two siblings with peroxisomal acyl-CoA oxidase deficiency. Brain and Development, 43(3), 475-481.

You, L., Chen, J., Liu, W., Xiang, Q., Luo, Z., Wang, W., ... & Wu, J. (2020). Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence, 11(1), 537-553.

Raas, Q., Saih, F. E., Gondcaille, C., Trompier, D., Hamon, Y., Leoni, V., ... & Savary, S. (2019). A microglial cell model for acyl-CoA oxidase 1 deficiency. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1864(4), 567-576.

Chen, X. F., Tian, M. X., Sun, R. Q., Zhang, M. L., Zhou, L. S., Jin, L., ... & Ye, D. (2018). SIRT 5 inhibits peroxisomal ACOX 1 to prevent oxidative damage and is downregulated in liver cancer. EMBO reports, 19(5), e45124.

Kim, S., & Kim, K. J. (2018). Structural insight into the substrate specificity of acyl-CoA oxidase1 from Yarrowia lipolytica for short-chain dicarboxylyl-CoAs. Biochemical and biophysical research communications, 495(2), 1628-1634.

Vamecq, J., Andreoletti, P., El Kebbaj, R., Saih, F. E., Latruffe, N., El Kebbaj, M., ... & Cherkaoui-Malki, M. (2018). Peroxisomal acyl-coA oxidase type 1: anti-inflammatory and anti-aging properties with a special emphasis on studies with LPS and argan oil as a model transposable to aging. Oxidative medicine and cellular longevity, 2018.

Zeng, J., Deng, S., Wang, Y., Li, P., Tang, L., & Pang, Y. (2017). Specific inhibition of Acyl-CoA oxidase-1 by an acetylenic acid improves hepatic lipid and reactive oxygen species (ROS) metabolism in rats fed a high fat diet. Journal of Biological Chemistry, 292(9), 3800-3809.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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