Rabbit Anti-ACOX1 Recombinant Antibody (V2-179360) (CBMAB-A0661-YC)

Name: Rabbit Anti-ACOX1 Recombinant Antibody (V2-179360)
SKU: CBMAB-A0661-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-179360

Application

IF, IHC, WB

Immunogen

Recombinant Human ACOX1 / AOX protein

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	1:100-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Oxidase 1

Introduction

ACOX1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in

Entrez Gene ID

UniProt ID

Q15067

Alternative Names

Acyl-CoA Oxidase 1; Palmitoyl-CoA Oxidase; Acyl-Coenzyme A Oxidase 1, Palmitoyl; Straight-Chain Acyl-CoA Oxidase; Acyl-CoA Oxidase 1, Palmitoyl; EC 1.3.3.6; ACOX;

Function

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. First enzyme of the fatty acid beta-oxidation pathway.
Isoform 1: Shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length.
Isoform 2: Is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.

Biological Process

Alpha-linolenic acid metabolic process
Cholesterol homeostasis
Fatty acid beta-oxidation using acyl-CoA oxidase
Fatty acid catabolic process
Fatty acid oxidation
Generation of precursor metabolites and energy
Hydrogen peroxide biosynthetic process
Lipid homeostasis
Lipid metabolic process
Peroxisome fission
Prostaglandin metabolic process
Protein localization
Regulation of metabolic process
Spermatogenesis
Very long-chain fatty acid beta-oxidation
Very long-chain fatty acid metabolic process

Cellular Location

Peroxisome

Involvement in disease

Adrenoleukodystrophy, pseudoneonatal (Pseudo-NALD): A peroxisomal single-enzyme disorder of fatty acid beta-oxidation, resulting in clinical manifestations that remind neonatal adrenoleukodystrophy. Clinical features include mental retardation, leukodystrophy, seizures, mild hepatomegaly, hearing deficit. Pseudo-NALD is characterized by increased plasma levels of very-long chain fatty acids, due to decreased or absent peroxisome acyl-CoA oxidase activity. Peroxisomes are intact and functioning.
Mitchell syndrome (MITCH): A disorder characterized by episodic demyelination, sensorimotor polyneuropathy, and sensorineural hearing loss.

Morita, A., Enokizono, T., Ohto, T., Tanaka, M., Watanabe, S., Takada, Y., ... & Takada, H. (2021). Novel ACOX1 mutations in two siblings with peroxisomal acyl-CoA oxidase deficiency. Brain and Development, 43(3), 475-481.

You, L., Chen, J., Liu, W., Xiang, Q., Luo, Z., Wang, W., ... & Wu, J. (2020). Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence, 11(1), 537-553.

Raas, Q., Saih, F. E., Gondcaille, C., Trompier, D., Hamon, Y., Leoni, V., ... & Savary, S. (2019). A microglial cell model for acyl-CoA oxidase 1 deficiency. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1864(4), 567-576.

Chen, X. F., Tian, M. X., Sun, R. Q., Zhang, M. L., Zhou, L. S., Jin, L., ... & Ye, D. (2018). SIRT 5 inhibits peroxisomal ACOX 1 to prevent oxidative damage and is downregulated in liver cancer. EMBO reports, 19(5), e45124.

Kim, S., & Kim, K. J. (2018). Structural insight into the substrate specificity of acyl-CoA oxidase1 from Yarrowia lipolytica for short-chain dicarboxylyl-CoAs. Biochemical and biophysical research communications, 495(2), 1628-1634.

Vamecq, J., Andreoletti, P., El Kebbaj, R., Saih, F. E., Latruffe, N., El Kebbaj, M., ... & Cherkaoui-Malki, M. (2018). Peroxisomal acyl-coA oxidase type 1: anti-inflammatory and anti-aging properties with a special emphasis on studies with LPS and argan oil as a model transposable to aging. Oxidative medicine and cellular longevity, 2018.

Zeng, J., Deng, S., Wang, Y., Li, P., Tang, L., & Pang, Y. (2017). Specific inhibition of Acyl-CoA oxidase-1 by an acetylenic acid improves hepatic lipid and reactive oxygen species (ROS) metabolism in rats fed a high fat diet. Journal of Biological Chemistry, 292(9), 3800-3809.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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