Rabbit Anti-CALD1 Recombinant Antibody (CBAb037) (CBMAB-Ab037-LY)

Name: Rabbit Anti-CALD1 Recombinant Antibody (CBAb037)
SKU: CBMAB-Ab037-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBAb037

Application

WB, IHC, IF, IP, FC

Immunogen

A synthesized peptide derived from human Caldesmon.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000-1:2,000
IHC	1:50-1:200
IF(ICC)	1:50-1:200
IP	1:50
FC	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 50% glycerol, 0.4-0.5mg/ml BSA

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Caldesmon 1

Introduction

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Entrez Gene ID

Human	800
Mouse	109624
Rat	25687

UniProt ID

Human	Q05682
Mouse	E9QA16
Rat	Q62736

Alternative Names

CALD1; CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22; caldesmon

Function

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Biological Process

Actin filament bundle assembly Source: GO_Central
Angiogenesis Source: GO_Central
Muscle contraction Source: Reactome

Cellular Location

Cytoskeleton; Stress fiber; Myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).

PTM

In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity).

Papke, D. J., Oliveira, A. M., Chou, M. M., & Fletcher, C. D. (2021). Morphologically malignant nodular fasciitis with CALD1-USP6 fusion. Virchows Archiv, 1-6.

Mamoor, S. (2021). CALD1 is differentially expressed in lymph node metastasis in human breast cancer.

Zheng, H., Bai, Y., Wang, J., Chen, S., Zhang, J., Zhu, J., ... & Wang, X. (2021). Weighted Gene Co-expression Network Analysis Identifies CALD1 as a Biomarker Related to M2 Macrophages Infiltration in Stage III and IV Mismatch Repair-Proficient Colorectal Carcinoma. Frontiers in molecular biosciences, 8.

Cheng, Q., Tang, A., Wang, Z., Fang, N., Zhang, Z., Zhang, L., ... & Zeng, Y. (2021). CALD1 Modulates Gliomas Progression via Facilitating Tumor Angiogenesis. Cancers, 13(11), 2705.

Śnit, M., Nabrdalik, K., Długaszek, M., Gumprecht, J., Trautsolt, W., Górczyńska-Kosiorz, S., & Grzeszczak, W. (2017). Association of rs 3807337 polymorphism of CALD1 gene with diabetic nephropathy occurrence in type 1 diabetes—preliminary results of a family-based study. Endokrynologia Polska, 68(1), 13-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CALD1 Matched Antibody Pair (169) (CAT#: APMAB-169LY)

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CALD1 Matched Antibody Pair (169) (CAT#: APMAB-169LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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