What applications is the Mouse Anti-CLEC2D Recombinant Antibody (CBXO-0384) recommended for?

The Mouse Anti-CLEC2D Recombinant Antibody (CBXO-0384) (CAT: CBMAB-O0131-CQ), targeting a cell surface protein, is expected to be suitable for applications such as Flow Cytometry (FCM) for detecting surface expression, Western Blotting (WB) for total protein levels, and potentially Immunofluorescence (IF) or Immunocytochemistry (ICC) for localization studies. For the most accurate and up-to-date information on validated applications and recommended working dilutions, please refer to the product datasheet provided by Creative Biolabs.

What is the host species and clonality of this antibody?

The Mouse Anti-CLEC2D Recombinant Antibody (CBXO-0384) is a recombinant monoclonal antibody produced in a Mouse host. Monoclonal antibodies provide high specificity and consistency due to their recognition of a single epitope.

Mouse Anti-CLEC2D Recombinant Antibody (CBXO-0384) (CBMAB-O0131-CQ)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXO-0384

Application

Immunogen

Amino acids 2-18 of OCIL of mouse.

Host Species

Mouse

Specificity

Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 57-191

More Infomation

Target

Full Name

C-Type Lectin Domain Family 2 Member D

Introduction

This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified for this gene.

Entrez Gene ID

93694

UniProt ID

Q91V08

Alternative Names

C-Type Lectin Domain Family 2 Member D; Lectin-Like Transcript 1; C-Type Lectin Superfamily 2, Member D; Lectin-Like NK Cell Receptor; Osteoclast Inhibitory Lectin; C-Type Lectin Related F

Function

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis (PubMed:20843815, PubMed:16339513).

Inhibits osteoclast formation (PubMed:14753741, PubMed:15123656).

Inhibits bone resorption (PubMed:14753741).

Modulates the release of interferon-gamma (PubMed:15104121).

Binds high molecular weight sulfated glycosaminoglycans (PubMed:15123656).

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Regulation of immune response Source: Reactome

Cellular Location

Cell membrane
Isoform 2&4: Endoplasmic reticulum

Topology

Cytoplasmic: 1-38
Helical: 39-59
Extracellular: 60-191

PTM

N-glycosylated.

Lai, J. J., Cruz, F. M., & Rock, K. L. (2020). Immune sensing of cell death through recognition of histone sequences by C-type lectin-receptor-2d causes inflammation and tissue injury. Immunity, 52(1), 123-135.

Del Fresno, C., & Sancho, D. (2020). Clec2d Joins the Cell Death Sensor Ranks. Immunity, 52(1), 6-8.

Marrufo, A. M., Mathew, S. O., Chaudhary, P., Malaer, J. D., Vishwanatha, J. K., & Mathew, P. A. (2018). Blocking LLT1 (CLEC2D, OCIL)-NKRP1A (CD161) interaction enhances natural killer cell-mediated lysis of triple-negative breast cancer cells. American journal of cancer research, 8(6), 1050.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CLEC2D Matched Antibody Pair (274) (CAT#: APMAB-274LY)

CLEC2D Matched Antibody Pair (275) (CAT#: APMAB-275LY)

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Mouse Anti-CLEC2D Recombinant Antibody (2E11) (CAT#: V2LY-1206-LY109)

CLEC2D Matched Antibody Pair (275) (CAT#: APMAB-275LY)

CLEC2D Matched Antibody Pair (274) (CAT#: APMAB-274LY)

Mouse Anti-CLEC2D Recombinant Antibody (CBFYC-1920) (CAT#: V2LY-1206-LY111)

Mouse Anti-CLEC2D Recombinant Antibody (4C7) (CAT#: V2LY-1206-LY110)

Mouse Anti-CLEC2D Recombinant Antibody (O0005) (CAT#: V2LY-1206-LY107)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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