Mouse Anti-CLEC2D Recombinant Antibody (4C7) (V2LY-1206-LY110)

Name: Mouse Anti-CLEC2D Recombinant Antibody (4C7)
SKU: V2LY-1206-LY110
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CLEC2D Recombinant Antibody (4C7) (V2LY-1206-LY110) in IHC

Immunohistochemical detection of LLT1 expression in RA ST cells.
To study surface-expressed LLT1 at the site of inflammation, 6 synovial tissue biopsies obtained from hand, shoulder or knee joints from long-standing, treated RA patients who underwent joint replacement surgery or synovectomy were processed for immunohistochemistry. Representative pictures showing immunohistochemical staining of consecutive tissue slides from 2 late-stage RA patients stained with antibodies against LLT1, CD68, CD3 and CD20cy (A,B). Graphs in C depict the results of the semiquantitative scoring (mean + SD) performed by 3 independent researchers. Scoring of the staining of all the markers was performed according to a 4-point scale: 0 = no positive cells; 1 = <5% positive cells; 2 = 5-50% positive cells; 3 =>50% positive cells. Three to five different pictures of each slide section (n = 6 different sections per biopsy) were taken. ...View More

Chalan, P., Bijzet, J., Huitema, M. G., Kroesen, B. J., Brouwer, E., & Boots, A. M. (2015). Expression of lectin-like transcript 1, the ligand for CD161, in rheumatoid arthritis. PLoS One, 10(7), e0132436.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CLEC2D Recombinant Antibody (4C7) (V2LY-1206-LY110) in WB

LLT1 protein detection by Western blot analysis of whole cell lysates of the indicated cell populations using 2F1 mAb. β-Actin was used as internal control. ...View More

Germain, C., Meier, A., Jensen, T., Knapnougel, P., Poupon, G., Lazzari, A., ... & Braud, V. M. (2011). Induction of lectin-like transcript 1 (LLT1) protein cell surface expression by pathogens and interferon-γ contributes to modulate immune responses. Journal of Biological Chemistry, 286(44), 37964-37975.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CLEC2D Recombinant Antibody (4C7) (V2LY-1206-LY110) in FC

Cell surface expression of CLEC2D protein isoforms was analyzed by flow cytometry on 293T cells or 293T-LLT1 cells transfected with CLEC2D isoforms or CLEC2A. ...View More

Germain, C., Bihl, F., Zahn, S., Poupon, G., Dumaurier, M. J., Rampanarivo, H. H., ... & Braud, V. M. (2010). Characterization of alternatively spliced transcript variants of CLEC2D gene. Journal of Biological Chemistry, 285(46), 36207-36215.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4C7

Application

ELISA, IHC-P, WB

Immunogen

Full-length recombinant CLEC2D.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-Type Lectin Domain Family 2 Member D

Entrez Gene ID

29121

UniProt ID

Q9UHP7

Function

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis (PubMed:20843815, PubMed:16339513).

Inhibits osteoclast formation (PubMed:14753741, PubMed:15123656).

Inhibits bone resorption (PubMed:14753741).

Modulates the release of interferon-gamma (PubMed:15104121).

Binds high molecular weight sulfated glycosaminoglycans (PubMed:15123656).

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Regulation of immune response Source: Reactome

Cellular Location

Cell membrane
Isoform 2&4: Endoplasmic reticulum

Topology

Cytoplasmic: 1-38
Helical: 39-59
Extracellular: 60-191

PTM

N-glycosylated.

Lai, J. J., Cruz, F. M., & Rock, K. L. (2020). Immune sensing of cell death through recognition of histone sequences by C-type lectin-receptor-2d causes inflammation and tissue injury. Immunity, 52(1), 123-135.

Del Fresno, C., & Sancho, D. (2020). Clec2d Joins the Cell Death Sensor Ranks. Immunity, 52(1), 6-8.

Marrufo, A. M., Mathew, S. O., Chaudhary, P., Malaer, J. D., Vishwanatha, J. K., & Mathew, P. A. (2018). Blocking LLT1 (CLEC2D, OCIL)-NKRP1A (CD161) interaction enhances natural killer cell-mediated lysis of triple-negative breast cancer cells. American journal of cancer research, 8(6), 1050.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CLEC2D Matched Antibody Pair (274) (CAT#: APMAB-274LY)

CLEC2D Matched Antibody Pair (275) (CAT#: APMAB-275LY)

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CLEC2D Matched Antibody Pair (274) (CAT#: APMAB-274LY)

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Mouse Anti-CLEC2D Recombinant Antibody (CBFYC-1920) (CAT#: V2LY-1206-LY111)

CLEC2D Matched Antibody Pair (275) (CAT#: APMAB-275LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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