PRAG1

PRAG1 is an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA).

Full Name

PEAK1 related, kinase-activating pseudokinase 1

Function

Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity).
Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity).
Acts as a critical coactivator of Notch signaling (By similarity).

Biological Process

Cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of neuron projection developmentISS:UniProtKB
Positive regulation of Rho protein signal transductionISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of cell motilityISS:UniProtKB
Regulation of cell shapeISS:UniProtKB
Regulation of Notch signaling pathwayISS:UniProtKB

Cellular Location

Cytoplasm
Cell junction, focal adhesion
Nucleus
Colocalized with NOTCH1 in the nucleus.

PTM

Phosphorylated by CSK on Tyr-253, Tyr-365, and Tyr-413; Tyr-413 is a primary site of phosphorylation.