STAT1 Antibodies

Structure of STAT1

STAT1 is a key signal transducer and transcriptional activator, with a molecular weight of approximately 91 kDa (STAT1α) and 84 kDa (STAT1β), and the molecular weights of different shear variants vary. This protein is composed of 751 amino acids and adopts a unique four-domain architecture: the amino-terminal domain, the coiled-coil domain, the DNA-binding domain and the SH2 domain. The secondary structure of STAT1 is mainly composed of an α -helix, among which the SH2 domain can recognize the phosphorylated tyrosine site and mediate protein dimerization. The dimerized STAT1 binds to the γ -interferon activation sequence (GAS) through the DNA binding domain, thereby initiating the transcription of downstream genes and playing a core role in the interferon signaling pathway and immune regulation.

Fig. 1 Schematic representation of STAT1 domains.¹

Key structural properties of STAT1:

• Four-domain architecture (N-terminal, Coiled-coil, DNA-binding and SH2 domains)
• SH2 domain structure recognition mediated phosphorylated tyrosine and two polymerization
• DNA binding domain specific recognition of interferon-gamma activation sequence (GAS)

Functions of STAT1

The main function of the STAT1 gene is to mediate cytokine signal transduction and immune gene regulation. In addition, it is also involved in processes such as cell proliferation, apoptosis regulation, and tumor suppression.

The activation curve of STAT1 shows a rapid response characteristic and forms a positive feedback loop with other signaling molecules in the Jak-STAT pathway, indicating its core position in the acute immune response.

Applications of STAT1 and STAT1 Antibody in Literature

1. Butturini, Elena, Alessandra Carcereri de Prati, and Sofia Mariotto. "Redox regulation of STAT1 and STAT3 signaling." International journal of molecular sciences 21.19 (2020): 7034. https://doi.org/10.3390/ijms21197034

The article indicates that STAT1 and STAT3 are transcription factors that regulate the cell cycle, survival and immune response, and are often activated by the same cytokines but exert antagonistic effects. This article reviews the mechanisms by which both achieve activity regulation through post-translational modification from the perspective of REDOX.

2. Tolomeo, Manlio, Andrea Cavalli, and Antonio Cascio. "STAT1 and its crucial role in the control of viral infections." International Journal of Molecular Sciences 23.8 (2022): 4095. https://doi.org/10.3390/ijms23084095

The article indicates that STAT1 is a core transcription factor mediating interferon signaling, activating the expression of various antiviral genes, and is crucial for resisting viruses such as Ebola and SARS-CoV-2. The absence of STAT1 can lead to fatal infections, and its function is also inhibited by multiple viral escape mechanisms. Therefore, the development of drugs targeting the STAT1 pathway has significant therapeutic significance.

3. Tehrani, Sahar SH, et al. "STAT1 is required to establish but not maintain interferon‐γ‐induced transcriptional memory." The EMBO Journal 42.14 (2023): e112259. https://doi.org/10.15252/embj.2022112259

The article indicates that STAT1 is a key factor in establishing interferon γ -induced transcriptional memory. It keeps the GBP gene in a "ready" state. Although it does not directly inherit memory, it enables STAT1 and IRF1 to bind to chromatin more quickly during secondary stimulation, thereby accelerating gene reactivation. This process does not rely on changes in the upstream JAK-STAT signal.

4. Li, et al. "The dual role of STAT1 in ovarian cancer: insight into molecular mechanisms and application potentials." Frontiers in Cell and Developmental Biology 9 (2021): 636595. https://doi.org/10.3389/fcell.2021.636595

The article indicates that STAT1 plays a dual role in ovarian cancer, both inhibiting and promoting cancer. It affects tumor development by regulating processes such as angiogenesis, proliferation, migration and drug resistance. This article focuses on reviewing the interaction between STAT1 and signaling pathways such as TGF-β, as well as their multifaceted molecular mechanisms and biological functions in ovarian cancer.

5. Bodmer, Daniel, et al. "STAT1 deficiency predisposes to spontaneous otitis media." PloS one 15.9 (2020): e0239952. https://doi.org/10.1371/journal.pone.0239952

The article indicates that STAT1 deficiency increases the susceptibility of otitis media in mice, leading to bilateral or unilateral hearing impairment. Histological findings revealed thickening of the middle ear mucosa and inflammatory exudation in STAT1 knockout mice, with the degree of inflammation associated with an elevated auditory brainstem response threshold. Studies have shown that STAT1 is a new related gene for susceptibility to otitis media.

Creative Biolabs: STAT1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality STAT1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom STAT1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our STAT1 antibodies, custom preparations, or technical support, contact us at email.